Suspension of Amorphous Calcium Phosphate Nanoparticles Impact Commitment of Human Adipose-Derived Stem Cells In Vitro

Amorphous calcium phosphate (aCaP) nanoparticles may trigger the osteogenic commitment of adipose-derived stem cells (ASCs) in vitro. The ASCs three human donors are investigated using basal culture medium DMEM to either 5 or 50 µg/mL aCaP suspension (control: no nanoparticles). After 7 14 days, cell marker genes, as well endothelial, osteogenic, chondrogenic, and adipogenic analyzed by qPCR. Free ion concentrations assessed supernatant. one week aCaP, downregulation markers ALP Runx2 is found, averaged across donors. Our results show that after two weeks, further downregulated, but upregulated. Endothelial such CD31 CD34, upregulated with a 2-week exposure. Inter-donor variability high: Two out significant upregulation at day compared aCaP. Notably, all changes obtained absence an medium. While chemical composition saturation status towards phases remain approximately same for conditions, gene expression considerably. Hence, potential endothelial ASCs.